Limited Phytochemical Screening

Oct 13, 2025 by miodeya.com 65 views

Antibacterial Activity of Ethanol Extract and Morbesi-Besi Leaf Fraction (Tarenna polycarpa) on Several Bacteria

Introduction

Morbesi-Besi leaves, derived from plants in the Rubiaceae family, have been widely recognized for their numerous health benefits. These leaves are often used as an antidiabetic drug, cholesterol-lowering agent, anti-inflammatory, and antibacterial. This study focuses on the antibacterial activity of ethanol extract and morbesi-besi leaf fraction on various types of bacteria. The aim of this study is to investigate the potential of morbesi-besi leaves as a natural antibacterial agent and to explore its mechanism and effectiveness.

Background

Morbesi-besi leaves contain various important chemical compounds, such as flavonoids, glycosides, steroids/triterpenoids, antasquinone, saponins, and tannins. These compounds have been reported to possess antibacterial, anti-inflammatory, and antioxidant properties. The presence of these compounds in morbesi-besi leaves makes it a potential source of natural antibacterial agents.

Materials and Methods

In this study, a phytochemical screening test was conducted on leaf powder, ethanol extract, N-Hexane fraction, ethyl acetate fraction, and the remaining fraction. The diffusion method was used to measure antibacterial activity by looking at the diameter of the clear zone formed around the disk paper. The test results were analyzed to determine the antibacterial activity of each fraction.

Results

The test results show that ethanol extract and ethyl acetate fraction have the highest antibacterial activity, with a concentration of inhibition of 500 mg/ml. Inhibition diameter for ethanol extract to Salmonella typhi bacteria reaches 26.8 mm, while the ethyl acetate fraction shows the inhibition diameter of 27.4 mm to the staphylococcus aureus. Conversely, the N-Hexane fraction and the remaining fraction showed a lower inhibitory activity than the two fractions, with a diameter of 22.7 mm inhibition for Staphylococcus epidermidis and 12.4 mm for propionibacterium acnes.

Phytochemical Screening

Testing phytochemical screening in ethanol extract shows the presence of steroid/triterpenoid compounds, glycosides, flavonoids, saponins, antasquinone, and tannin. Meanwhile, the N-Hexane fraction identified contains steroid compounds and triterpenoids, which are supported by TLC results that show 5.9 stains after being sprayed with H2SO4 and 7 stains by spraying Liebermann-Burchard.

Discussion

The results of this study suggest that morbesi-besi leaves (Tarenna polycarpa) have the potential as a source of natural antibacterial agents. The presence of various active compounds in morbesi-besi leaves makes it a promising candidate for further research. The antibacterial activity of ethanol extract and ethyl acetate fraction suggests that these fractions may be used as a natural antibacterial agent in the treatment of bacterial infections.

Conclusion

In conclusion, this study has demonstrated the antibacterial activity of ethanol extract and morbesi-besi leaf fraction on several bacteria. The results of this study suggest that morbesi-besi leaves have the potential as a source of natural antibacterial agents. Further research is needed to explore the mechanism and effectiveness of the extract and fractions produced. This study also opened up opportunities for further research on other potentials of morbesi-besi leaves in the treatment of diseases and public health.

Future Directions

Further research is needed to explore the mechanism and effectiveness of the extract and fractions produced. This study also opened up opportunities for further research on other potentials of morbesi-besi leaves in the treatment of diseases and public health. Some potential areas of research include:

Mechanism of Action: Further research is needed to understand the mechanism of action of morbesi-besi leaves as an antibacterial agent.
Effectiveness: Further research is needed to determine the effectiveness of morbesi-besi leaves in the treatment of bacterial infections.
Other Potentials: Further research is needed to explore the other potentials of morbesi-besi leaves in the treatment of diseases and public health.

Limitations

This study has some limitations that need to be addressed in future research. Some of the limitations include:

Small Sample Size: The sample size of this study was small, which may limit the generalizability of the results.
Limited Bacterial Strains: This study only tested the antibacterial activity of morbesi-besi leaves on a limited number of bacterial strains.
Limited Phytochemical Screening: This study only conducted a limited phytochemical screening of the extract and fractions produced.

Recommendations

Based on the results of this study, the following recommendations are made:

Further Research: Further research is needed to explore the mechanism and effectiveness of the extract and fractions produced.
Large-Scale Studies: Large-scale studies are needed to determine the effectiveness of morbesi-besi leaves in the treatment of bacterial infections.
Other Potentials: Further research is needed to explore the other potentials of morbesi-besi leaves in the treatment of diseases and public health.
Q&A: Antibacterial Activity of Ethanol Extract and Morbesi-Besi Leaf Fraction (Tarenna polycarpa)

Frequently Asked Questions

This article aims to provide answers to some of the most frequently asked questions about the antibacterial activity of ethanol extract and morbesi-besi leaf fraction (Tarenna polycarpa).

Q: What is Morbesi-Besi Leaf?

A: Morbesi-besi leaf is a type of leaf derived from plants in the Rubiaceae family. It has been widely recognized for its numerous health benefits, including antibacterial, anti-inflammatory, and antioxidant properties.

Q: What is the Antibacterial Activity of Morbesi-Besi Leaf?

A: The antibacterial activity of morbesi-besi leaf has been demonstrated in several studies, including this one. The ethanol extract and ethyl acetate fraction of morbesi-besi leaf have been shown to have the highest antibacterial activity, with a concentration of inhibition of 500 mg/ml.

Q: What are the Active Compounds in Morbesi-Besi Leaf?

A: The active compounds in morbesi-besi leaf include flavonoids, glycosides, steroids/triterpenoids, antasquinone, saponins, and tannins. These compounds have been reported to possess antibacterial, anti-inflammatory, and antioxidant properties.

Q: How Does Morbesi-Besi Leaf Work as an Antibacterial Agent?

A: The exact mechanism of action of morbesi-besi leaf as an antibacterial agent is not fully understood. However, it is believed that the active compounds in morbesi-besi leaf interact with the bacterial cell membrane, disrupting its structure and function, and ultimately leading to the death of the bacterial cell.

Q: Is Morbesi-Besi Leaf Safe for Human Consumption?

A: While morbesi-besi leaf has been shown to have numerous health benefits, its safety for human consumption has not been fully established. Further research is needed to determine the safety and efficacy of morbesi-besi leaf as a dietary supplement.

Q: Can Morbesi-Besi Leaf Be Used to Treat Bacterial Infections?

A: While morbesi-besi leaf has been shown to have antibacterial activity, its effectiveness in treating bacterial infections has not been fully established. Further research is needed to determine the efficacy of morbesi-besi leaf as a treatment for bacterial infections.

Q: What are the Potential Applications of Morbesi-Besi Leaf?

A: The potential applications of morbesi-besi leaf are numerous and include:

Antibacterial Agent: Morbesi-besi leaf can be used as an antibacterial agent to treat bacterial infections.
Dietary Supplement: Morbesi-besi leaf can be used as a dietary supplement to promote overall health and well-being.
Cosmetic Ingredient: Morbesi-besi leaf can be used as a cosmetic ingredient to promote skin health and reduce the appearance of fine lines and wrinkles.

Q: What are the Limitations of This Study?

A: This study has several limitations, including:

Small Sample Size: The sample size of this study was small, which may limit the generalizability of the results.
Limited Bacterial Strains: This study only tested the antibacterial activity of morbesi-besi leaf on a limited number of bacterial strains.
Limited Phytochemical Screening: This study only conducted a limited phytochemical screening of the extract and fractions produced.

Q: What are the Future Directions of This Research?

A: The future directions of this research include:

Large-Scale Studies: Large-scale studies are needed to determine the effectiveness of morbesi-besi leaf in the treatment of bacterial infections.
Other Potentials: Further research is needed to explore the other potentials of morbesi-besi leaf in the treatment of diseases and public health.
Mechanism of Action: Further research is needed to understand the mechanism of action of morbesi-besi leaf as an antibacterial agent.