Analysis of Effects on Hepar Histology

Oct 13, 2025 by miodeya.com 23 views

The Effect of Giving a Mixture of Tuak and Arilus Durian (Durio Zibethinus Murr.) Against the Hepar Histology and the Leukocyte Value of Mice (Mus Musculus L.) Male

Introduction

Tuak, a fermented drink derived from palm sap, contains about 4% alcohol. This study aims to investigate the effect of giving a mixture of tuak and arilus durian (Durio Zibethinus Murr.) on liver histology and leukocyte values in male mice (Mus Musculus L.). The study employed a Complete Randomized Design (RAL) with four treatments and seven replications. The treatments consisted of controls (K0) given aquades, P1 given wine, P2 given Arilus durian mixed with distilled water, and P3 that gets a mixture of tuak and arilus durian. Each treatment was given as much as 0.3 ml.

Materials and Methods

The preparation of the liver organs was carried out using the paraffin block and coloring method with Hematoxylin erlich-eosin (HE). Furthermore, leukocyte analysis was carried out using an automated hematology analyzer tool that works based on the principle of flow cytometry. The results showed that the histology of the level of hepatocyte damage and the basophil value did not show a significant difference (P> 0.05) for hepatocyte damage. However, there are significant differences (p <0.05) in the basophil value. Meanwhile, there are significant differences (p <0.05) in the value of lymphocytes, neutrophils, eusinophils, and basophils between the control group and the treatment group.

Analysis of Effects on Hepar Histology

The results of this study indicate that the administration of wine and arilus durian has a variety of impacts on the histology of the liver mice. Although hepatocyte damage does not show significant differences, it is important to notice that there is an increase in the basophil value. Basophils are a type of white blood cells involved in the response of allergies and inflammation. This increase can indicate a more active immune reaction in response to the treatment given.

On the other hand, a significant difference in the value of lymphocytes, neutrophils, and eusinophils shows that both materials have the potential to affect the immune system of mice. Lymphocytes play an important role in the body's defense against infection, while neutrophils and eusinophils are involved in the response to various types of infections and allergic reactions. Therefore, further monitoring of how tuak and Arilus Durian affect the immunological activity of mice need to be done to get a deeper understanding of the mechanisms involved.

Discussion

The results of this study suggest that the administration of wine and arilus durian has a significant impact on the immune system of mice. The increase in basophil value indicates a more active immune reaction, while the significant difference in the value of lymphocytes, neutrophils, and eusinophils shows that both materials have the potential to affect the immune system. This finding can be a foundation for further research on the effects of natural ingredients such as palm wine and durian in the context of liver health and immune systems.

Conclusion

In this study, it can be concluded that giving a mixture of palm wine and arilus durian affects the value of leukocytes and liver histology in male mice. Although there is no significant difference in hepatocyte damage, changes in leukocyte values indicate an influence on the immune system. This finding can be a foundation for further research on the effects of natural ingredients such as palm wine and durian in the context of liver health and immune systems. People can consider these results in utilizing natural food in their diet, while continuing to conduct research to ensure the safety and benefits.

Recommendations

Based on the findings of this study, the following recommendations can be made:

Further research is needed to understand the mechanisms involved in the effects of tuak and arilus durian on the immune system of mice.
The safety and benefits of using natural ingredients such as palm wine and durian in the context of liver health and immune systems need to be further investigated.
The use of tuak and arilus durian as a natural remedy for liver health and immune system disorders should be approached with caution and further research is needed to ensure its safety and efficacy.

Limitations

This study has several limitations that need to be addressed in future research. These include:

The study was conducted on male mice only, and further research is needed to understand the effects of tuak and arilus durian on female mice.
The study only investigated the effects of tuak and arilus durian on liver histology and leukocyte values, and further research is needed to understand the effects on other organs and systems.
The study only used a small sample size, and further research is needed to confirm the findings and understand the mechanisms involved.

Future Directions

Based on the findings of this study, the following future directions can be suggested:

Further research is needed to understand the mechanisms involved in the effects of tuak and arilus durian on the immune system of mice.
The safety and benefits of using natural ingredients such as palm wine and durian in the context of liver health and immune systems need to be further investigated.
The use of tuak and arilus durian as a natural remedy for liver health and immune system disorders should be approached with caution and further research is needed to ensure its safety and efficacy.

Conclusion

In conclusion, this study provides evidence that giving a mixture of tuak and arilus durian affects the value of leukocytes and liver histology in male mice. Although there is no significant difference in hepatocyte damage, changes in leukocyte values indicate an influence on the immune system. This finding can be a foundation for further research on the effects of natural ingredients such as palm wine and durian in the context of liver health and immune systems.
Q&A: The Effect of Giving a Mixture of Tuak and Arilus Durian (Durio Zibethinus Murr.) Against the Hepar Histology and the Leukocyte Value of Mice (Mus Musculus L.) Male

Q: What is tuak and how is it related to this study?

A: Tuak is a fermented drink derived from palm sap and contains about 4% alcohol. In this study, tuak was mixed with arilus durian (Durio Zibethinus Murr.) and given to male mice (Mus Musculus L.) to investigate its effects on liver histology and leukocyte values.

Q: What is arilus durian and how is it related to this study?

A: Arilus durian is a type of fruit that is native to Southeast Asia. In this study, arilus durian was mixed with tuak and given to male mice (Mus Musculus L.) to investigate its effects on liver histology and leukocyte values.

Q: What were the main findings of this study?

A: The main findings of this study were that giving a mixture of tuak and arilus durian affected the value of leukocytes and liver histology in male mice. Although there was no significant difference in hepatocyte damage, changes in leukocyte values indicated an influence on the immune system.

Q: What are the implications of this study?

A: The implications of this study are that tuak and arilus durian may have a potential impact on the immune system of mice. Further research is needed to understand the mechanisms involved and to confirm the findings.

Q: What are the limitations of this study?

A: The limitations of this study include the small sample size and the fact that it was conducted on male mice only. Further research is needed to confirm the findings and to understand the effects on female mice.

Q: What are the future directions of this study?

A: The future directions of this study include further research to understand the mechanisms involved in the effects of tuak and arilus durian on the immune system of mice. Additionally, the safety and benefits of using natural ingredients such as palm wine and durian in the context of liver health and immune systems need to be further investigated.

Q: Can tuak and arilus durian be used as a natural remedy for liver health and immune system disorders?

A: While the findings of this study suggest that tuak and arilus durian may have a potential impact on the immune system of mice, it is not recommended to use them as a natural remedy for liver health and immune system disorders without further research and confirmation.

Q: What are the potential risks of using tuak and arilus durian as a natural remedy?

A: The potential risks of using tuak and arilus durian as a natural remedy include the possibility of adverse effects on the liver and immune system. Further research is needed to understand the safety and efficacy of using these ingredients as a natural remedy.

Q: How can the findings of this study be applied to human health?

A: The findings of this study can be applied to human health by further investigating the effects of tuak and arilus durian on the liver and immune system in humans. Additionally, the safety and benefits of using natural ingredients such as palm wine and durian in the context of liver health and immune systems need to be further investigated.

Q: What are the next steps in this research?

A: The next steps in this research include further investigation of the mechanisms involved in the effects of tuak and arilus durian on the immune system of mice. Additionally, the safety and benefits of using natural ingredients such as palm wine and durian in the context of liver health and immune systems need to be further investigated.